The CycloGraph system is a centrifugally accelerated device for performing preparative thin layer radial separations. The separations occur quickly (typically within 20 minutes) with no need for scraping separated bands as with traditional Thin Layer Chromatography.
Principle of Operation: The compound to be separated is applied as a solution inside the adsorbent ring of the pre-cast Rotor (Analtechs prepared consumable for this device) by way of a solvent pump or hand held syringe.
The chosen solvent mixture (mobile phase) is then pumped onto the center of the spinning Rotor, passing through the adsorbent layer effectively separating the individual components by centrifugally acceelerated chromatographic action (a result of their different affinities for the layer and solvent mixture). As the individual rings reach the outer rim of the Rotor they are spun off the edge of the glass and collected in the specially designed circular trough.
The angle of the trough allows the eluent to collect at the bottom and exit at the collection port.